Hydrophilic interaction chromatography is a separation technique suitable for polar and hydrophilic compounds and orthogonal to reversed phase liquid chromatography from small organic molecules to proteins the text explores the many applications of hilic in the analytical field. Hydrophilic interaction liquid chromatography hilic and advanced applications 1st edition perry g wang weixuan he may 22 2017 this is the first book that comprehensively and systematically describes the new technology of hydrophilic interaction liquid chromatography hilic. 10212 hydrophilic interaction liquid chromatography in contrast to rplc and hic hydrophilic interaction liquid chromatography hilic uses a hydrophilic or polar stationary phase and a mobile phase with high organic content and gradient increasing in polar content thus more hydrophobic species will elute first. Hydrophilic interaction chromatography or hydrophilic interaction liquid chromatography hilic is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography. Since alpert coined the term hydrophilic interaction liquid chromatography hilic to define the highly efficient chromatographic separation of very polar molecules that takes place through the interaction between an aqueous organic mobile phase and a polar stationary phase the technique has been fine tuned and accounts of many of its applications have been published
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